LOCUS pSLQ7334: pHR-PGK-SV40_NLS-dCas12f-VPR-c- Myc_NLS-mCherry-WPRE 13028 bp ds-DNA circular SYN 01-OCT-2021
DEFINITION Expression of dCas12f-VPR-mCherry in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pSLQ7334: pHR-PGK-SV40_NLS-dCas12f-VPR-c- Myc_NLS-mCherry-WPRE
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13028)
AUTHORS Xu X, Chemparathy A, Zeng L, Kempton HR, Shang S, Nakamura M, Qi LS
TITLE Engineered miniature CRISPR-Cas system for mammalian genome
regulation and editing.
JOURNAL Mol Cell. 2021 Aug 26. pii: S1097-2765(21)00648-1. doi:
10.1016/j.molcel.2021.08.008.
PUBMED 34480847
REFERENCE 2 (bases 1 to 13028)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Oct 1, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..13028
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 27..144
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 209..708
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(244..267)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 641..660
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
regulatory 768..777
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 780..800
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 2400..2426
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 2478..2627
/codon_start=1
/product="tetrameric repeat of the minimal activation
domain of herpes simplex virus VP16 (Beerli et al., 1998)"
/label=VP64
/translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
DLDML"
CDS 3072..3443
/codon_start=1
/product="transcriptional activation domain of human RelA,
also known as p65 (O'Shea and Perkins, 2008)"
/label=RelA (p65) AD
/translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
EDFSSIADMDFSALLSQISS"
CDS 3462..4031
/codon_start=1
/product="transcriptional activation domain from the human
herpesvirus 4 (Epstein-Barr virus) replication and
transcription activator Rta/BRLF1 (Hardwick et al., 1992;
Chavez et al., 2015)"
/label=Rta AD
/translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
DTFLNDECLLHAMHISTGLSIFDTSLF"
CDS 4059..4085
/codon_start=1
/product="nuclear localization signal of human c-Myc
proto-oncogene (Dang and Lee, 1988)"
/label=c-myc NLS
/translation="PAAKRVKLD"
CDS 4098..4805
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
GRHSTGGMDELYK"
primer_bind complement(4240..4258)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 4508..4527
/label=mCherry-F
/note="mCherry, forward primer"
misc_feature 4869..5457
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4922..4942)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5340..5351)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(5460..5476)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5461..5477)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 5567..5800
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter complement(5825..5843)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(5826..5843)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(6133..6152)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(6366..6385)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6485..6507
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6545..6563)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6631..6735
/gene="bla"
/label=AmpR promoter
CDS 6736..7596
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6954..6973)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7767..8355
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8256..8275
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8509..8526
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(8596..8616)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 8601..8930
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 8781..8916
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8843..8862
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 10064..10129
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 10259..10279
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 10704..10838
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10741..10760)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10795..10814
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 11006..11639
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 11686..11811
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 12308..12541
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 12726..12770
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
ORIGIN
1 cgccaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
61 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
121 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
181 atcgataagc ttgatatcga attctaccgg gtaggggagg cgcttttccc aaggcagtct
241 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
301 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
361 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
421 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
481 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
541 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
601 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
661 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca
721 agcttacgcg tctgcaggat atcaagcttg cggtaccgcg ggcccgggcc accatgggac
781 ccaagaaaaa acgcaaggtg ggaagcggat ccgccaaaaa caccattacc aaaacactga
841 aactgcgtat tgtgcgtccg tataatagcg cagaagtgga aaaaattgtt gccgacgaaa
901 aaaacaaccg cgaaaaaatc gcactggaaa agaacaaaga caaagtgaaa gaagcctgca
961 gcaaacatct gaaagttgca gcatattgta ccacacaggt tgaacgtaat gcatgcctgt
1021 tttgtaaagc acgtaaactg gatgacaaat tctaccaaaa actgcgtggt cagtttccgg
1081 atgcagtttt ttggcaagaa atcagcgaaa tttttcgcca gctgcagaaa caggcagcag
1141 aaatctataa tcagagcctg atcgaactgt actacgagat ttttatcaaa ggcaaaggta
1201 ttgcaaatgc cagcagcgtt gaacattatc tgagtgatgt ttgttatacc cgtgcagcag
1261 aactgtttaa aaacgcagca attgcaagcg gtctgcgtag caaaatcaaa agcaattttc
1321 gtctgaaaga actgaaaaac atgaaaagtg gtctgccgac caccaaaagc gataattttc
1381 cgattccgct ggttaaacag aaaggtggtc agtataccgg ttttgaaatt agcaatcata
1441 atagcgactt catcatcaag attccgtttg gtcgttggca ggtcaaaaaa gagattgata
1501 aatatcgtcc gtgggagaaa tttgactttg aacaggttca gaaaagcccg aaaccgatta
1561 gcctgctgct gagcacccag cgtcgtaaac gtaataaagg ttggagcaaa gatgaaggca
1621 ccgaagccga aatcaaaaaa gttatgaatg gcgattatca gaccagctac attgaagtta
1681 aacgtggcag caaaatctgt gaaaaaagcg catggatgct gaatctgagc attgatgttc
1741 cgaaaattga taaaggtgtg gatccgagca ttattggtgg tattgcagtt ggtgttaaat
1801 caccgctggt ttgcgcaatt aacaatgcat ttagccgtta tagcatcagc gataacgacc
1861 tgtttcactt caacaagaaa atgtttgcac gtcgtcgtat cctgctgaaa aaaaaccgtc
1921 ataaacgtgc aggtcatggt gcaaaaaaca aactgaaacc gatcaccatt ctgaccgaaa
1981 aaagtgaacg ttttcgcaaa aagctgattg aacgttgggc atgtgaaatc gcggatttct
2041 tcattaaaaa caaagttggc accgtgcaga tggaaaatct ggaaagcatg aaacgtaaag
2101 aggacagcta ttttaacatt cgcctgcgtg gcttttggcc gtatgcagaa atgcagaaca
2161 aaatcgaatt caaactgaag cagtatggca tcgaaattcg taaagttgca ccgaataata
2221 ccagcaaaac ctgtagcaaa tgtggccatc tgaacaacta tttcaacttc gagtaccgca
2281 agaaaaacaa attcccgcac tttaaatgcg aaaaatgcaa cttcaaagaa aacgccgcgt
2341 ataatgcagc cctgaatatt tcaaacccga aactgaaaag caccaaagag gaaccggcct
2401 atccctatga cgtgcccgat tatgccagcc tgggcagcgg cgacggcatt ggtagtggga
2461 gcaacggcag cagcctcgat gctttagacg attttgactt agatatgctt ggttcagacg
2521 cgttagacga cttcgaccta gacatgttag gctcagatgc attggacgac ttcgatttag
2581 atatgttggg ctccgatgcc ctagatgact ttgatctaga tatgctaggt agtggcggca
2641 gcggatccca gtatctgccc gacacagatg atagacaccg aatcgaagag aaacgcaagc
2701 gaacgtatga aaccttcaaa tcgatcatga agaaatcgcc cttctcgggt ccgaccgatc
2761 ccaggccccc accgagaagg attgcggtcc cgtcccgctc gtcggccagc gtgccgaagc
2821 ctgcgccgca gccctacccc ttcacgtcga gcctgagcac aatcaattat gacgagttcc
2881 cgacgatggt gttcccctcg ggacaaatct cacaagcctc ggcgctcgca ccagcgcctc
2941 cccaagtcct tccgcaagcg cctgccccag cgcctgcacc ggcaatggtg tccgccctcg
3001 cacaggcccc tgcgcccgtc cccgtgctcg cgcctggacc gccccaggcg gtcgctccac
3061 cggctccgaa gccgacgcag gccggagagg gaacactctc cgaagcactt cttcaactcc
3121 agtttgatga cgaggatctt ggagcactcc ttggaaactc gacagaccct gcggtgttta
3181 ccgacctcgc gtcagtagat aactccgaat ttcagcagct tttgaaccag ggtatcccgg
3241 tcgcgccaca tacaacggag cccatgttga tggaataccc cgaagcaatc acgagacttg
3301 tgacgggagc gcagcggcct cccgatcccg cacccgcacc tttgggggca cctggcctcc
3361 ctaacggact tttgagcggc gacgaggatt tctcctccat cgccgatatg gatttctcag
3421 ccttgctgtc acagatttcc agcggctctg gcagcggcag ccgggattcc agggaaggga
3481 tgtttttgcc gaagcctgag gccggctccg ctattagtga cgtgtttgag ggccgcgagg
3541 tgtgccagcc aaaacgaatc cggccatttc atcctccagg aagtccatgg gccaaccgcc
3601 cactccccgc cagcctcgca ccaacaccaa ccggtccagt acatgagcca gtcgggtcac
3661 tgaccccggc accagtccct cagccactgg atccagcgcc cgcagtgact cccgaggcca
3721 gtcacctgtt ggaggatccc gatgaagaga cgagccaggc tgtcaaagcc cttcgggaga
3781 tggccgatac tgtgattccc cagaaggaag aggctgcaat ctgtggccaa atggaccttt
3841 cccatccgcc cccaaggggc catctggatg agctgacaac cacacttgag tccatgaccg
3901 aggatctgaa cctggactca cccctgaccc cggaattgaa cgagattctg gataccttcc
3961 tgaacgacga gtgcctcttg catgccatgc atatcagcac aggactgtcc atcttcgaca
4021 catctctgtt tcatatgggt ggaggctccg gggaagatcc tgctgccaaa cgcgttaaac
4081 tagacatggg aagcggagtg agcaagggcg aggaggataa catggccatc atcaaggagt
4141 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
4201 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
4261 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
4321 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
4381 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
4441 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
4501 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
4561 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
4621 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
4681 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
4741 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
4801 agtaagcggc cgcataagac tctagagtcg acctgcaggc atgcaagctt gatatcaagc
4861 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
4921 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
4981 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
5041 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
5101 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
5161 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
5221 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
5281 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
5341 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
5401 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
5461 gataccgtcg acctcgaggg aattaattcg agctcggtac ctttaagacc aatgacttac
5521 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
5581 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
5641 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
5701 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
5761 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt
5821 gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt
5881 agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc
5941 agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct
6001 gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca
6061 tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
6121 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
6181 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
6241 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
6301 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact
6361 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
6421 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
6481 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
6541 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
6601 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
6661 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
6721 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
6781 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
6841 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
6901 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
6961 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
7021 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
7081 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
7141 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
7201 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
7261 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
7321 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
7381 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
7441 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
7501 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
7561 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
7621 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
7681 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
7741 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
7801 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
7861 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
7921 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
7981 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
8041 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
8101 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
8161 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
8221 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
8281 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
8341 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
8401 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
8461 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
8521 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
8581 cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
8641 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
8701 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
8761 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
8821 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
8881 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac
8941 acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc
9001 agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat
9061 aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg
9121 acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt
9181 aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt
9241 ggcggtctgt accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg
9301 tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag
9361 aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg
9421 ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg
9481 gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc
9541 cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc
9601 tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt
9661 attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc
9721 tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct
9781 gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg
9841 atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg
9901 atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc
9961 ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt
10021 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
10081 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
10141 tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc
10201 agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc
10261 aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt
10321 gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga
10381 aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag
10441 gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc
10501 tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt
10561 taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
10621 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
10681 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
10741 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
10801 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
10861 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
10921 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
10981 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
11041 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
11101 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
11161 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
11221 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
11281 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
11341 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
11401 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
11461 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
11521 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
11581 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
11641 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
11701 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
11761 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
11821 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
11881 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
11941 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
12001 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
12061 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
12121 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
12181 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
12241 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
12301 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
12361 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
12421 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
12481 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
12541 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
12601 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
12661 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
12721 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
12781 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
12841 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
12901 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
12961 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
13021 gacggtat
//
